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Addgene

iSM_PPL2-IP
(Plasmid #235989)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235989 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEF1a-IP
  • Backbone size w/o insert (bp) 6278
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iSM_PPL2
  • Alt name
    iSpyMac with Spy-Cas9 PID loop 2
  • Species
    Synthetic
  • Mutation
    replacement of 5 amino acids of Smac-Cas9 PL2 with 16 amino acids from Spy-Cas9 PL2
  • Promoter EF-1a

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    iSM_PPL2-IP was a gift from Tobias Cantz (Addgene plasmid # 235989 ; http://n2t.net/addgene:235989 ; RRID:Addgene_235989)

  • For your References section:

    PAM-interacting domain turn-helix 51 motifs can improve Cas9-SpRY activity. Eggenschwiler R, Hoffmann T, Dmytrenko O, Opitz M, Ackel-Zakour M, Wang P, McCallan SA, Fraguas-Eggenschwiler M, Niemann H, Patronov A, Beisel CL, Cantz T. Nucleic Acids Res. 2025 Aug 11;53(15):gkaf782. doi: 10.1093/nar/gkaf782. 10.1093/nar/gkaf782 PubMed 40808297
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