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Addgene

SpRY_SlTH51-IP
(Plasmid #235993)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235993 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEF1a-IP
  • Backbone size w/o insert (bp) 6278
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpRY_ATH51
  • Alt name
    Cas9-SpRY with PID turn-helix 51 motif from S. lutetiensis Cas9
  • Species
    Synthetic
  • Mutation
    ectopic insertion of 8 amino acids of PID turn-helix 51 from S. lutetiensis Cas9
  • Promoter EF-1a

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SpRY_SlTH51-IP was a gift from Tobias Cantz (Addgene plasmid # 235993 ; http://n2t.net/addgene:235993 ; RRID:Addgene_235993)

  • For your References section:

    PAM-interacting domain turn-helix 51 motifs can improve Cas9-SpRY activity. Eggenschwiler R, Hoffmann T, Dmytrenko O, Opitz M, Ackel-Zakour M, Wang P, McCallan SA, Fraguas-Eggenschwiler M, Niemann H, Patronov A, Beisel CL, Cantz T. Nucleic Acids Res. 2025 Aug 11;53(15):gkaf782. doi: 10.1093/nar/gkaf782. 10.1093/nar/gkaf782 PubMed 40808297
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