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PB-PE iSM_PPL2
(Plasmid #235996)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235996 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    PB-PE
  • Backbone manufacturer
    Dr. Reto Eggenschwiler
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology ; piggyBac transposon

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    iSM_PPL2_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
  • Alt name
    iSM_PPL2 prime editor
  • Species
    Synthetic
  • Mutation
    replacement of 5 amino acids of Smac-Cas9 PL2 with 16 amino acids from Spy-Cas9 PL2
  • Tags / Fusion Proteins
    • bpSV40 NLS (N terminal on insert)
    • bpSV40 NLS (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Can be integrated into genome using hyPBase. Contains both, prime editor and U6 promoter driven site for insertion of pegRNA. Can be employed for transient expression experiments, but in case pegRNA is added on a separate plasmid, removal of U6 in PB-PE vector is recommended.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-PE iSM_PPL2 was a gift from Tobias Cantz (Addgene plasmid # 235996 ; http://n2t.net/addgene:235996 ; RRID:Addgene_235996)

  • For your References section:

    PAM-interacting domain turn-helix 51 motifs can improve Cas9-SpRY activity. Eggenschwiler R, Hoffmann T, Dmytrenko O, Opitz M, Ackel-Zakour M, Wang P, McCallan SA, Fraguas-Eggenschwiler M, Niemann H, Patronov A, Beisel CL, Cantz T. Nucleic Acids Res. 2025 Aug 11;53(15):gkaf782. doi: 10.1093/nar/gkaf782. 10.1093/nar/gkaf782 PubMed 40808297
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