pET19b-pps-UvsX
(Plasmid
#236046)
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PurposepET19b-pps-UvsX
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236046 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 * |
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Backbone
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Vector backbonepET19 pps
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Modifications to backboneprecission protease at the N-terminal
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameUvsX
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Alt nameUvsX
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SpeciesSynthetic; bacteriophage T4
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Insert Size (bp)1188
- Promoter T7 RNA polymerase
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Tag
/ Fusion Protein
- His tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer t7 promoter (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET19b-pps-UvsX was a gift from Luis Gabriel Brieba (Addgene plasmid # 236046 ; http://n2t.net/addgene:236046 ; RRID:Addgene_236046)