pET19pps-EndoIV(Thermus Thermophilus)
(Plasmid
#236048)
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PurposepET19pps-APE1(Thermus Thermophilus)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236048 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 * |
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Backbone
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Vector backbonepET19 pss
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Modifications to backboneprecission protease after the Histidine tag
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameEndo IV from Thermus thermophilus
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Alt nameEndoIV Thermus thermophilus
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SpeciesSynthetic; Thermus thermophilus
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Insert Size (bp)813
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GenBank IDNC_006461.1:c800688-799876 NC_006461.1:c800688-799876
- Promoter T7 RNA polymerase
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Tags
/ Fusion Proteins
- His tag (N terminal on backbone)
- Histidine tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer t7 promoter (Common Sequencing Primers)
Terms and Licenses
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET19pps-EndoIV(Thermus Thermophilus) was a gift from Luis Gabriel Brieba (Addgene plasmid # 236048 ; http://n2t.net/addgene:236048 ; RRID:Addgene_236048)