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pET19pps-EndoIV(Thermus Thermophilus)
(Plasmid #236048)

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Item Catalog # Description Quantity Price (USD)
Plasmid 236048 Standard format: Plasmid sent in bacteria as agar stab 1 $89 *
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Backbone

  • Vector backbone
    pET19 pss
  • Modifications to backbone
    precission protease after the Histidine tag
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Endo IV from Thermus thermophilus
  • Alt name
    EndoIV Thermus thermophilus
  • Species
    Synthetic; Thermus thermophilus
  • Insert Size (bp)
    813
  • GenBank ID
    NC_006461.1:c800688-799876 NC_006461.1:c800688-799876
  • Promoter T7 RNA polymerase
  • Tags / Fusion Proteins
    • His tag (N terminal on backbone)
    • Histidine tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer t7 promoter
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET19pps-EndoIV(Thermus Thermophilus) was a gift from Luis Gabriel Brieba (Addgene plasmid # 236048 ; http://n2t.net/addgene:236048 ; RRID:Addgene_236048)

  • For your References section:

    The concentration of single-stranded DNA-binding proteins is a critical factor in recombinase polymerase amplification (RPA), as revealed by insights from an open-source system. Cordoba-Andrade F, Peralta-Castro A, Garcia-Medel PL, Castro-Torres E, Gonzalez-Gonzalez R, Castro-Lara AY, Mora Garduno JD, Raygoza CD, Baruch-Torres N, Penafiel-Ayala A, Diaz-Quezada C, Cardona-Felix CS, Guzman Chavez F, Trasvina-Arenas CH, Sotelo-Mundo RR, Xoconostle-Cazares B, Martinez-Antonio A, Brieba de Castro LG. PeerJ. 2025 Aug 13;13:e19758. doi: 10.7717/peerj.19758. eCollection 2025. 10.7717/peerj.19758 PubMed 40821985
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