pGlomyc-Grhl1 (618 aa)
(Plasmid #236049)

• Purpose: Mammalian expression vector with myc-tagged mouse Grhl1 coding sequence (long isoform)
• Depositing Lab: Renee van Amerongen
• Publication: GRHL and PGR control WNT4 expression in the mammary gland via 3D looping of conserved and species-specific enhancers (unpublished)

Backbone

• Vector backbone: pGlomyc (based on pCDNA3.1)
• Backbone manufacturer: Olivier Destree
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7500
• Modifications to backbone: The pGlomyc vector backbone contains 5’ and 3’ globin UTRs and the pCDNA1.1 BamHI-NsiI polylinker in the backbone of pCDNA3.1(+).
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Grhl1
• Species: M. musculus (mouse)
• Insert Size (bp): 1800
• Entrez Gene: Grhl1 (a.k.a. LBP-32, MGR, Tcfcp2l2)
• Promoter: CMV
• Tag / Fusion Protein:
  • myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: insert sequence was ordered by Gene Block from IDT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry