pAD120-CHIP-G132N-myc-His
(Plasmid
#236053)
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PurposeMammalian expression of human CHIP with the synthetic G132N substitution and C-terminal myc-His tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236053 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepcDNA3.1/myc-His
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCHIP-G132N
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Alt nameSTUB1
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Alt nameSTIP1 homology and U-box containing protein 1
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SpeciesH. sapiens (human)
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MutationG132N
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Entrez GeneSTUB1 (a.k.a. CHIP, HSPABP2, NY-CO-7, SCA48, SCAR16, SDCCAG7, UBOX1)
- Promoter CMV
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Tag
/ Fusion Protein
- myc-His (C terminal on insert)
Cloning Information
- Cloning method Other
- 5′ sequencing primer T7
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAD120-CHIP-G132N-myc-His was a gift from Jonathan Schisler (Addgene plasmid # 236053 ; http://n2t.net/addgene:236053 ; RRID:Addgene_236053) -
For your References section:
Phosphorylation-State Modulated Binding of HSP70: Structural Insights and Compensatory Protein Engineering. Stewart M, Paththamperuma C, McCann C, Cottingim K, Zhang H, DelVecchio R, Peng I, Fennimore E, Nix JC, Saeed MN, George K, Makaroff M, Colie M, Paulakonis E, Almeida MF, Afolayan AJ, Brown NG, Page RC, Schisler JC. bioRxiv 2025.02.17.637997 10.1101/2025.02.17.637997