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Addgene

pSmBit-CHIP-K30A
(Plasmid #236066)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236066 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBiT2.1-N
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SmBit-CHIP-K30A
  • Alt name
    STUB1
  • Alt name
    STIP1 homology and U-box containing protein 1
  • Species
    H. sapiens (human)
  • Mutation
    K30A
  • Entrez Gene
    STUB1 (a.k.a. CHIP, HSPABP2, NY-CO-7, SCA48, SCAR16, SDCCAG7, UBOX1)
  • Promoter HSV-TK
  • Tag / Fusion Protein
    • SmBit (N terminal on insert)

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSmBit-CHIP-K30A was a gift from Jonathan Schisler (Addgene plasmid # 236066 ; http://n2t.net/addgene:236066 ; RRID:Addgene_236066)

  • For your References section:

    Phosphorylation-State Modulated Binding of HSP70: Structural Insights and Compensatory Protein Engineering. Stewart M, Paththamperuma C, McCann C, Cottingim K, Zhang H, DelVecchio R, Peng I, Fennimore E, Nix JC, Saeed MN, George K, Makaroff M, Colie M, Paulakonis E, Almeida MF, Afolayan AJ, Brown NG, Page RC, Schisler JC. bioRxiv 2025.02.17.637997 10.1101/2025.02.17.637997
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