PB{3xP3-dsRed2; β2Tubulin-mNeonGreen}
(Plasmid
#236085)
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PurposePiggyBac plasmid for the insertion of a sperm-expressed green fluorescent protein in Aedes aegypti
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 236085 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePiggyBac
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Modifications to backboneThis plasmid was created through replacement of the fluorescent proteins in the original PB{3xP3-EGFP; β2Tubulin-dsRed2} plasmid (Addgene #236086)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name3xP3-dsRed2; β2Tubulin-mNeonGreen
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB{3xP3-dsRed2; β2Tubulin-mNeonGreen} was a gift from Leslie Vosshall (Addgene plasmid # 236085 ; http://n2t.net/addgene:236085 ; RRID:Addgene_236085)
