Skip to main content
Addgene

POC1358
(Plasmid #236103)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236103 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    POC1355
  • Backbone manufacturer
    Self-made
  • Backbone size w/o insert (bp) 4357
  • Total vector size (bp) 5269
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DNA fragment flanked by BsaI sites
  • Alt name
    MICA gene fragment modified
  • Insert Size (bp)
    912

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (unknown if destroyed)
  • 3′ cloning site BsaI (unknown if destroyed)
  • 5′ sequencing primer TGGTGTAAACAAATTGACGC
  • 3′ sequencing primer ACGCCCTTTTAAATATCCG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    POC1358 was a gift from Christopher A. O'Callaghan (Addgene plasmid # 236103 ; http://n2t.net/addgene:236103 ; RRID:Addgene_236103)