pTK41
(Plasmid #236110)

• Purpose: Expresses the motor domain of rat KIF5C fused with SNAP tag and ALFA tag nanobody
• Depositing Lab: Shinsuke Niwa
• Publication: Kita et al Cell Rep Phys Sci, 6 (2): 102440. 19 Feb 2025.

Backbone

• Vector backbone: pET32a
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: KIF5C(C7S)(1-430)-SNAP-His
• Species: R. norvegicus (rat)
• Mutation: C7S mutation is introduced. 1-430 aa is encoded.
• Entrez Gene: Kif5c
• Promoter: T7
• Tags / Fusion Proteins:
  • SNAP tag (C terminal on insert)
  • His tag (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: none

Gene/Insert 2

• Gene/Insert name: KIF5C(C7S)(1-430)-ALFA tag nanobody-StrepII tag
• Species: R. norvegicus (rat)
• Mutation: C7S mutation is introduced. 1-430 aa is encoded.
• Entrez Gene: Kif5c
• Promoter: T7
• Tags / Fusion Proteins:
  • ALFA tag nanobody (C terminal on insert)
  • StrepII tag (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: none

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Ken'ya Furuta and Junichiro Yajima

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

KIF5C(C7S)(1-430) was originally published in following papers.
https://doi.org/10.1016/S0960-9822(01)00683-2
https://doi.org/10.1073/pnas.1201390110

We changed one of the tags into ALFA tag nanobody.

For your Reference section:
Modular photostable fluorescent DNA blocks dissect the effects of pathogenic mutant kinesin on collective transport.
T Kita, R Sugie, Y Suzuki, S Niwa. Cell Reports Physical Science 2025
https://doi.org/10.1016/j.xcrp.2025.102440