eDHFR-E3
(Plasmid
#236113)
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PurposeHalf of chemically inducible GFE3. When co-expressed with 236112 can degrade inhibitory synapses in presence of TH. (trimethoprim-HaloTag ligand).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236113 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameeDHFR-E3
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.09.23.614589 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
eDHFR-E3 was a gift from Don Arnold (Addgene plasmid # 236113 ; http://n2t.net/addgene:236113 ; RRID:Addgene_236113) -
For your References section:
A toolbox for ablating excitatory and inhibitory synapses. Bareghamyan A, Deng C, Daoudi S, Yadav SC, Lu X, Zhang W, Campbell RE, Kramer RH, Chenoweth DM, Arnold DB. eLife. 2025 Apr 29;13:RP103757. doi: 10.7554/eLife.103757. 10.7554/eLife.103757 PubMed 40298577