FLAG-FBXL17
(Plasmid
#236188)
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PurposeExpression of N-terminal tagged FLAG-FBXL17 (H. sapiens, codon-optimized) in human cell lines.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236188 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
- Total vector size (bp) 8158
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFLAG-FBXL17
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SpeciesH. sapiens (human)
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Insert Size (bp)2139
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Entrez GeneFBXL17 (a.k.a. FBXO13, Fbl17, Fbx13)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-FBXL17 was a gift from Michele Pagano (Addgene plasmid # 236188 ; http://n2t.net/addgene:236188 ; RRID:Addgene_236188) -
For your References section:
Recognition of BACH1 quaternary structure degrons by two F-box proteins under oxidative stress. Cao S, Garcia SF, Shi H, James EI, Kito Y, Shi H, Mao H, Kaisari S, Rona G, Deng S, Goldberg HV, Ponce J, Ueberheide B, Lignitto L, Guttman M, Pagano M, Zheng N. Cell. 2024 Oct 30:S0092-8674(24)01188-7. doi: 10.1016/j.cell.2024.10.012. 10.1016/j.cell.2024.10.012 PubMed 39504958