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Addgene

IMPT-11371
(Plasmid #236191)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236191 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac1
  • Backbone manufacturer
    gibco
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GPR119
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2709
  • Entrez Gene
    GPR119 (a.k.a. GPCR2)
  • Promoter polyhedrin

Cloning Information

  • Cloning method Gene Synthesis
  • 5′ sequencing primer GGATTATTCATACCGTCCCA
  • 3′ sequencing primer CAAATGTGGTATGGCTGATT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IMPT-11371 was a gift from Vadim Cherezov (Addgene plasmid # 236191 ; http://n2t.net/addgene:236191 ; RRID:Addgene_236191)

  • For your References section:

    Native mass spectrometry prescreening of G protein-coupled receptor complexes for cryo-EM structure determination. Kim D, Liu W, Viner R, Cherezov V. Structure. 2024 Dec 5;32(12):2206-2219.e4. doi: 10.1016/j.str.2024.10.004. Epub 2024 Oct 28. 10.1016/j.str.2024.10.004 PubMed 39471802
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