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MSCV-IRES-NUP98::NSD1-GFP
(Plasmid #236201)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236201 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV-IRES-GFP
  • Backbone size w/o insert (bp) 6492
  • Total vector size (bp) 12195
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NUP98::NSD1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5703
  • Entrez Gene
    NUP98 (a.k.a. ADIR2, NUP196, NUP96, Nup98-96)
  • Entrez Gene
    NSD1 (a.k.a. ARA267, KMT3B, SOTOS, SOTOS1, STO)
  • Promoter MSCV

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CTTTATCCAGCCCTCAC
  • 3′ sequencing primer GCATTCCTTTGGCGAGAG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The NUP98::NSD1 original plasmid was received from the University of Veterinary Medicine 103 Vienna, Austria, as described in Schmoellerl et al and cloned into MIG backbone.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-IRES-NUP98::NSD1-GFP was a gift from Michael Heuser (Addgene plasmid # 236201 ; http://n2t.net/addgene:236201 ; RRID:Addgene_236201)

  • For your References section:

    Clec12a is required for the pathogenesis of NUP98::NSD1 AML. Mohanty S, Charles Cano F, Gabdoulline R, Lai CK, Othman B, Sudarsanam H, Eder T, Grebien F, Lipka DB, Henschler R, Heuser M. Blood Adv. 2025 May 7:bloodadvances.2024015739. doi: 10.1182/bloodadvances.2024015739. 10.1182/bloodadvances.2024015739 PubMed 40334081
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