MSCV-IRES-NUP98::NSD1-GFP
(Plasmid #236201)

• Purpose: Expresses NUP98::NSD1 fusion protein in mammalian cells
• Depositing Lab: Michael Heuser
• Publication: Mohanty et al Blood Adv. 2025 May 7:bloodadvances.2024015739. doi: 10.1182/bloodadvances.2024015739.

Backbone

• Vector backbone: MSCV-IRES-GFP
• Backbone size w/o insert (bp): 6492
• Total vector size (bp): 12195
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NUP98::NSD1
• Species: H. sapiens (human)
• Insert Size (bp): 5703
• Entrez Gene: NUP98 (a.k.a. ADIR2, NUP196, NUP96, Nup98-96)
• Entrez Gene: NSD1 (a.k.a. ARA267, KMT3B, SOTOS, SOTOS1, STO)
• Promoter: MSCV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CTTTATCCAGCCCTCAC
• 3′ sequencing primer: GCATTCCTTTGGCGAGAG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The NUP98::NSD1 original plasmid was received from the University of Veterinary Medicine 103 Vienna, Austria, as described in Schmoellerl et al and cloned into MIG backbone.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry