pEB0_11_5xQUAS_CYC1cp
(Plasmid
#236215)
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PurposePlasmid encoding 5xQUAS_CYC1cp (five repeats of the binding sequence of QF directly upstream of the CYC1 core promoter) as a Type 2 part to be used in the Dueber Yeast MoClo Toolkit.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 236215 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneMoClo YTK (pYTK001)
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCYC1 core promoter with 5x QUAS binding sites
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SpeciesSynthetic
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bypMAL513, Lalwani et al. (2021).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid made are based on the Yeast MoClo Toolkit.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEB0_11_5xQUAS_CYC1cp was a gift from Yvonne Nygård (Addgene plasmid # 236215 ; http://n2t.net/addgene:236215 ; RRID:Addgene_236215) -
For your References section:
Expanding the Yeast MoClo Toolkit: gene expression control parts for Saccharomyces cerevisiae tested in industrially relevant conditions. Blick E, Nygard Y. Synth Biol (Oxf). 2025 Dec 4;10(1):ysaf018. doi: 10.1093/synbio/ysaf018. eCollection 2025. 10.1093/synbio/ysaf018 PubMed 41439119