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pEB2_14_Q-system-QF2-ctrl
(Plasmid #236220)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236220 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    MoClo YTK
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    QF2, ACT1 terminator
  • Species
    Synthetic
  • Promoter PGK1p

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    mTurquoise2, ADH1 terminator
  • Species
    Synthetic
  • Promoter 5xQUAS_CYC1cp

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pMAL513, Lalwani et al. (2021).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid made are based on the Yeast MoClo Toolkit.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEB2_14_Q-system-QF2-ctrl was a gift from Yvonne Nygård (Addgene plasmid # 236220 ; http://n2t.net/addgene:236220 ; RRID:Addgene_236220)

  • For your References section:

    Expanding the Yeast MoClo Toolkit: gene expression control parts for Saccharomyces cerevisiae tested in industrially relevant conditions. Blick E, Nygard Y. Synth Biol (Oxf). 2025 Dec 4;10(1):ysaf018. doi: 10.1093/synbio/ysaf018. eCollection 2025. 10.1093/synbio/ysaf018 PubMed 41439119
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