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pOET3-6xHis-hSMSr
(Plasmid #236224)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236224 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOET3
  • Backbone manufacturer
    Oxford Expression Technology
  • Backbone size w/o insert (bp) 4542
  • Total vector size (bp) 5790
  • Modifications to backbone
    6xHis tag are inserted
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SAMD8
  • Alt name
    Sphingomyelin synthase-related protein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1248
  • Mutation
    wild type
  • Entrez Gene
    SAMD8 (a.k.a. HEL-177, SMSr)
  • Tags / Fusion Proteins
    • Hexa histidine tag (6xHis-tag) (N terminal on insert)
    • enterokinase site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GCTGATATCGGGAGTTCAGTCGTCGAATGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOET3-6xHis-hSMSr was a gift from Chiaki Murakami (Addgene plasmid # 236224 ; http://n2t.net/addgene:236224 ; RRID:Addgene_236224)

  • For your References section:

    Sphingomyelin synthase-related protein generates diacylglycerol via the hydrolysis of glycerophospholipids in the absence of ceramide. Murakami C, Sakane F. J Biol Chem. 2021 Jan-Jun;296:100454. doi: 10.1016/j.jbc.2021.100454. Epub 2021 Feb 20. 10.1016/j.jbc.2021.100454 PubMed 33621517
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