pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV1.2 WPRE
(Plasmid
#236241)
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PurposeLentiviral expression of voltage-gated calcium channel, CaV1.2, N-terminally tagged with mEmerald
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236241 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSIV third-generation, simian immunodeficiency based, pro-lentiviral vector
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCaV1.2
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SpeciesO. cuniculus (rabbit)
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Insert Size (bp)7299
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Entrez GeneCACNA1C (a.k.a. CACB, CaV1.2)
- Promoter human Synapsin 1
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Tag
/ Fusion Protein
- mEmerald (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site HpaI (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV1.2 WPRE was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 236241 ; http://n2t.net/addgene:236241 ; RRID:Addgene_236241) -
For your References section:
Periodic ER-plasma membrane junctions support long-range Ca(2+) signal integration in dendrites. Benedetti L, Fan R, Weigel AV, Moore AS, Houlihan PR, Kittisopikul M, Park G, Petruncio A, Hubbard PM, Pang S, Xu CS, Hess HF, Saalfeld S, Rangaraju V, Clapham DE, De Camilli P, Ryan TA, Lippincott-Schwartz J. Cell. 2025 Jan 23;188(2):484-500.e22. doi: 10.1016/j.cell.2024.11.029. Epub 2024 Dec 20. 10.1016/j.cell.2024.11.029 PubMed 39708809