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Addgene

mE-GFP2A
(Plasmid #236247)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236247 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pDONOR221
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 3336
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eGFP
  • Alt name
    enhanced GFP
  • Alt name
    eGFP2A
  • Alt name
    eGFP-Viral2A
  • Insert Size (bp)
    1394
  • Mutation
    Added a viral2A sequence with no stop codon

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    GFP viral2A sequence was a kind gift from Steven Leach (Provost et al, 2007)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mE-GFP2A was a gift from James Amatruda & Genevieve Kendall (Addgene plasmid # 236247 ; http://n2t.net/addgene:236247 ; RRID:Addgene_236247)

  • For your References section:

    PAX3-FOXO1 transgenic zebrafish models identify HES3 as a mediator of rhabdomyosarcoma tumorigenesis. Kendall GC, Watson S, Xu L, LaVigne CA, Murchison W, Rakheja D, Skapek SX, Tirode F, Delattre O, Amatruda JF. eLife. 2018 Jun 5;7. pii: 33800. doi: 10.7554/eLife.33800. 10.7554/eLife.33800 PubMed 29869612
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