mE-mCherry2A
(Plasmid
#236248)
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PurposeMiddle entry fluorescent mCherry reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236248 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
- Total vector size (bp) 3308
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCherry2A
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Alt namemCherry viral2A
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Insert Size (bp)781
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MutationAdded a viral2A sequence to mCherry with no stop codon
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bymCherry viral2A sequence was a kind gift Steven Leach (Provost et al, 2007)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mE-mCherry2A was a gift from James Amatruda & Genevieve Kendall (Addgene plasmid # 236248 ; http://n2t.net/addgene:236248 ; RRID:Addgene_236248) -
For your References section:
PAX3-FOXO1 transgenic zebrafish models identify HES3 as a mediator of rhabdomyosarcoma tumorigenesis. Kendall GC, Watson S, Xu L, LaVigne CA, Murchison W, Rakheja D, Skapek SX, Tirode F, Delattre O, Amatruda JF. eLife. 2018 Jun 5;7. pii: 33800. doi: 10.7554/eLife.33800. 10.7554/eLife.33800 PubMed 29869612