pLV-Bsd-CMV>tTS/rtTA:T2A:{cymR}
(Plasmid #236251)

• Purpose: Blasticidin selected lentiviral vector with Tet-On regulatory proteins and the cumate repressor, for use with dual-inducible CymR-CuO system
• Depositing Lab: Genevieve Kendall
• Publication: Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10.1038/s41598-024-71227-3.

Backbone

• Vector backbone: Tet Regulatory Protein Expression Lentiviral Vector
• Backbone manufacturer: VectorBuilder
• Backbone size w/o insert (bp): 6800
• Total vector size (bp): 10106
• Modifications to backbone: Added a cymR sequence directly downstream of the tTS and rtTA proteins linked via a T2A sequence (linked by a second T2A sequence); added a blasticidin resistance gene for antibiotic selection
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: tTS/rtTA
• Species: Synthetic
• Insert Size (bp): 1653
• Mutation: rTS and rtTA_M2 linked by T2A
• Promoter: CMV

Cloning Information for Gene/Insert 1

• Cloning method: Gene Synthesis
• 5′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: cymR
• Alt name: cumate repressor
• Species: Synthetic
• Insert Size (bp): 612
• Promoter: CMV

Cloning Information for Gene/Insert 2

• Cloning method: Gene Synthesis
• 5′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • ptz1055 pLV-Bsd-CMV_tTS-rtTA-T2A-cymR.gb
• A portion of this plasmid was derived from a plasmid made by: VectorBuilder

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry