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Addgene

pETCK3 TGP-E
(Plasmid #236255)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236255 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETCK3
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Thermal Green Protein Q66E (TGP-E)
  • Species
    Synthetic
  • Insert Size (bp)
    756
  • Promoter lac operon
  • Tag / Fusion Protein
    • His tag (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    the Los Alamos National Lab. The original TGP insert was modified to include the Q66E mutation.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETCK3 TGP-E was a gift from Natasha DeVore (Addgene plasmid # 236255 ; http://n2t.net/addgene:236255 ; RRID:Addgene_236255)

  • For your References section:

    Green, yellow, or cyan? Introduction of color change mutations into a green thermostable fluorescent protein and characterization during an introduction to biochemistry lab course. Anderson MR, Dargatz CJ, Banerjee T, DeVore NM. Biochem Mol Biol Educ. 2024 Sep-Oct;52(5):549-558. doi: 10.1002/bmb.21841. Epub 2024 Jun 8. 10.1002/bmb.21841 PubMed 38850239
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