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pPtGE31
(Plasmid #236257)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236257 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCC1BAC
  • Backbone size (bp) 16334
  • Modifications to backbone
    This plasmid is derived from a pCC1BAC backbone. It contains an algal selection marker (NAT- nourseothricin resistance), origin of transfer (oriT) for conjugation, P. tricornutum region for episomal stability, as well as bacterial and yeast artificial chromosome elements. The algal marker is driven by the FcpD/FcpA promoter-terminator pair.
  • Vector type
    Bacterial Expression, Yeast Expression, Synthetic Biology ; P. tricornutum Expression
  • Selectable markers
    URA3, HIS3 ; Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Growth instructions
    This plasmid can be induced to high-copy number replication by supplementation with L-arabinose (100 µg/ml). Otherwise, it is maintained as 1-2 copies per cell.
  • Copy number
    Low Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPtGE31 was a gift from Bogumil Karas (Addgene plasmid # 236257 ; http://n2t.net/addgene:236257 ; RRID:Addgene_236257)
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