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Addgene

BtARR1 R175E
(Plasmid #236263)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236263 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTrcHisB
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 4400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    arrestin-1
  • Alt name
    visual arrestin
  • Alt name
    clone en1
  • Species
    B. taurus (bovine)
  • Mutation
    R175E, inserted GGT codon after starting ATG
  • Entrez Gene
    SAG (a.k.a. S-AG)
  • Promoter lac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer M13_pUC_rev_primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The arrestin coding sequence was cloned into the pTrcHisB vector between NcoI and HindIII sites, removing the His tag.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BtARR1 R175E was a gift from Vsevolod Gurevich (Addgene plasmid # 236263 ; http://n2t.net/addgene:236263 ; RRID:Addgene_236263)
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