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Addgene

BtARR1 Cys-less
(Plasmid #236274)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236274 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTrcHisB
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 4400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    arrestin-1
  • Alt name
    visual arrestin
  • Alt name
    clone eNCL
  • Species
    B. taurus (bovine)
  • Mutation
    C63A, C128S, C143A, inserted GGT codon after starting ATG
  • Entrez Gene
    SAG (a.k.a. S-AG)
  • Promoter lac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco RI (unknown if destroyed)
  • 3′ cloning site Hind III (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The arrestin coding sequence was cloned into the pTrcHisB vector between NcoI and HindIII sites, removing the His tag.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BtARR1 Cys-less was a gift from Vsevolod Gurevich (Addgene plasmid # 236274 ; http://n2t.net/addgene:236274 ; RRID:Addgene_236274)

  • For your References section:

    Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Hanson SM, Francis DJ, Vishnivetskiy SA, Kolobova EA, Hubbell WL, Klug CS, Gurevich VV. Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):4900-5. doi: 10.1073/pnas.0600733103. Epub 2006 Mar 17. 10.1073/pnas.0600733103 PubMed 16547131
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