pL(CMVtight-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE)
(Plasmid
#236278)
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PurposeLentiviral vector that can express doxycycline-inducible hAqp1 with fkbp12 degron in Tet ON mammalian cells. CMVtight promoter. EGFP marker.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236278 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonelentiviral vector
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman aquaporin 1
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SpeciesH. sapiens (human)
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Entrez GeneAQP1 (a.k.a. AQP-CHIP, CHIP28, CO)
- Promoter CMVtight
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Tags
/ Fusion Proteins
- fkbp12 (C terminal on insert)
- Flag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer gaagaaggtggagagag (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pL(CMVtight-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE) was a gift from Arnab Mukherjee (Addgene plasmid # 236278 ; http://n2t.net/addgene:236278 ; RRID:Addgene_236278) -
For your References section:
Destabilized reporters for background-subtracted, chemically-gated, and multiplexed deep-tissue imaging. Yun J, Huang Y, Miller ADC, Chang BL, Baldini L, Dhanabalan KM, Li E, Li H, Mukherjee A. Chem Sci. 2024 Jun 4;15(28):11108-11121. doi: 10.1039/d4sc00377b. eCollection 2024 Jul 17. 10.1039/d4sc00377b PubMed 39027298