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pYSGDLuc
(Plasmid #236297)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236297 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGREG-505
  • Backbone size (bp) 11102
  • Vector type
    Yeast Expression
  • Promoter TEF1
  • Selectable markers
    LEU2 ; G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Renilla and Firefly luciferase are codon optimized for expression in S. cerevisiae. Renilla, test sequence and Firefly products are separated by StopGo (E2A) sequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYSGDLuc was a gift from Pasha Baranov (Addgene plasmid # 236297 ; http://n2t.net/addgene:236297 ; RRID:Addgene_236297)

  • For your References section:

    Comprehensive analysis of yeast +1 ribosomal frameshifting unveils a novel stimulator supporting two distinct frameshifting mechanisms. Fenton DA, Bozko M, Swirski MI, Loughran G, Yordanova MM, Kufel J, Atkins JF, Baranov PV. Nucleic Acids Res. 2025 Nov 26;53(22):gkaf1301. doi: 10.1093/nar/gkaf1301. 10.1093/nar/gkaf1301 PubMed 41312768
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