HSPD1-i7
-HT2
(Plasmid
#236333)
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PurposeInducibly expresses HSPD1 tagged with HaloTag2 at the relative location of intron 7 in the HSPD1 gene.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 236333 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneCustom
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHSPD1
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SpeciesH. sapiens (human)
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Entrez GeneHSPD1 (a.k.a. CPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13)
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Tag
/ Fusion Protein
- HaloTag2 insertion at intron 7
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HSPD1-i7 -HT2 was a gift from Ophir Shalem (Addgene plasmid # 236333 ; http://n2t.net/addgene:236333 ; RRID:Addgene_236333) -
For your References section:
Pooled tagging and hydrophobic targeting of endogenous proteins for unbiased mapping of unfolded protein responses. Sansbury SE, Serebrenik YV, Lapidot T, Burslem GM, Shalem O. bioRxiv. 2023 Jul 14:2023.07.13.548611. doi: 10.1101/2023.07.13.548611. Preprint. 10.1101/2023.07.13.548611 PubMed 37503003