pHAGE-EFS-Halo-NLS-tDeg
(Plasmid
#236370)
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PurposeOverexpression of Halo-NLS-tDeg in human cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236370 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHAGE-EF1a-IRES-puro
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHalo-NLS-tDeg
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SpeciesSynthetic
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAGE-EFS-Halo-NLS-tDeg was a gift from Ling-Ling Chen (Addgene plasmid # 236370 ; http://n2t.net/addgene:236370 ; RRID:Addgene_236370) -
For your References section:
CRISPR-array-mediated imaging of non-repetitive and multiplex genomic loci in living cells. Yang LZ, Min YH, Liu YX, Gao BQ, Liu XQ, Huang Y, Wang H, Yang L, Liu ZJ, Chen LL. Nat Methods. 2024 Sep;21(9):1646-1657. doi: 10.1038/s41592-024-02333-3. Epub 2024 Jul 4. 10.1038/s41592-024-02333-3 PubMed 38965442