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pLIC-His6-MBP-TEV-Rgs14
(Plasmid #236395)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236395 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLIC-MBP
  • Backbone manufacturer
    John Sondek (University of North Carolina)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rattus norvegicus regulator of G-protein signaling 14
  • Alt name
    Rgs14
  • Species
    R. norvegicus (rat)
  • GenBank ID
    U92279.1
  • Entrez Gene
    Rgs14
  • Tags / Fusion Proteins
    • hexahistidine (H6) tag (N terminal on insert)
    • maltose-binding protein (MBP) tag (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    D.P. Siderovski (REF: B E Snow, L Antonio, S Suggs, H B Gutstein, D P Siderovski (1997) Molecular cloning and expression analysis of rat Rgs12 and Rgs14. Biochem Biophys Res Commun. Apr 28;233(3):770-7. doi: 10.1006/bbrc.1997.6537. PMID: 9168931)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIC-His6-MBP-TEV-Rgs14 was a gift from John Hepler (Addgene plasmid # 236395 ; http://n2t.net/addgene:236395 ; RRID:Addgene_236395)

  • For your References section:

    Integration of G protein alpha (Galpha) signaling by the regulator of G protein signaling 14 (RGS14). Brown NE, Goswami D, Branch MR, Ramineni S, Ortlund EA, Griffin PR, Hepler JR. J Biol Chem. 2015 Apr 3;290(14):9037-49. doi: 10.1074/jbc.M114.634329. Epub 2015 Feb 9. 10.1074/jbc.M114.634329 PubMed 25666614
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