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Addgene

pcDNA3.1(+)-FLAG-Rgs14
(Plasmid #236396)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236396 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1+
  • Backbone manufacturer
    Thermo Fisher (Invitrogen)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rattus norvegicus regulator of G-protein signaling 14
  • Alt name
    Rgs14
  • Species
    R. norvegicus (rat)
  • GenBank ID
    U92279.1
  • Entrez Gene
    Rgs14
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    D.P. Siderovski (REF: B E Snow, L Antonio, S Suggs, H B Gutstein, D P Siderovski (1997) Molecular cloning and expression analysis of rat Rgs12 and Rgs14. Biochem Biophys Res Commun. Apr 28;233(3):770-7. doi: 10.1006/bbrc.1997.6537. PMID: 9168931)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)-FLAG-Rgs14 was a gift from John Hepler (Addgene plasmid # 236396 ; http://n2t.net/addgene:236396 ; RRID:Addgene_236396)

  • For your References section:

    Selective interactions between Gi alpha1 and Gi alpha3 and the GoLoco/GPR domain of RGS14 influence its dynamic subcellular localization. Shu FJ, Ramineni S, Amyot W, Hepler JR. Cell Signal. 2007 Jan;19(1):163-76. doi: 10.1016/j.cellsig.2006.06.002. Epub 2006 Jul 25. 10.1016/j.cellsig.2006.06.002 PubMed 16870394
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