pH-EF1α-DualPE
(Plasmid
#236417)
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PurposeFor plant prime editing including large DNA fragment editing in Solanum lycopersicum or other dicotyledons
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236417 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepH-2×35S-DualPE
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Vector typePlant Expression, CRISPR
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEF1α promoter
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SpeciesSolanum lycopersicum L.
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Insert Size (bp)1582
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pH-EF1α-DualPE was a gift from Zong Yuan (Addgene plasmid # 236417 ; http://n2t.net/addgene:236417 ; RRID:Addgene_236417) -
For your References section:
Precise deletion, replacement and inversion of large DNA fragments in plants using dual prime editing. Zhao Y, Huang Z, Zhou X, Teng W, Liu Z, Wang W, Tang S, Liu Y, Liu J, Wang W, Chai L, Zhang N, Guo W, Liu J, Ni Z, Sun Q, Wang Y, Zong Y. Nat Plants. 2025 Feb;11(2):191-205. doi: 10.1038/s41477-024-01898-3. Epub 2025 Jan 13. 10.1038/s41477-024-01898-3 PubMed 39805934