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Addgene

pcDNA3.1 2xFLAG-2xSTREP-Keap1 (mouse)
(Plasmid #236421)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236421 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KEAP1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Keap1 (a.k.a. INRF2, mKIAA0132)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 2xFLAG-2xSTREP-Keap1 (mouse) was a gift from Michele Pagano (Addgene plasmid # 236421 ; http://n2t.net/addgene:236421 ; RRID:Addgene_236421)
  • For your References section:

    EMSY inhibits homologous recombination repair and the interferon response, promoting lung cancer immune evasion. Marzio A, Kurz E, Sahni JM, Di Feo G, Puccini J, Jiang S, Hirsch CA, Arbini AA, Wu WL, Pass HI, Bar-Sagi D, Papagiannakopoulos T, Pagano M. Cell. 2022 Jan 6;185(1):169-183.e19. doi: 10.1016/j.cell.2021.12.005. Epub 2021 Dec 27. 10.1016/j.cell.2021.12.005 PubMed 34963055