pcDNA3.1 2xFLAG-2xSTREP-Keap1 (mouse)
(Plasmid
#236421)
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Purposetransient overexpression of KEAP1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236421 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKEAP1
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SpeciesM. musculus (mouse)
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Entrez GeneKeap1 (a.k.a. INRF2, mKIAA0132)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1 2xFLAG-2xSTREP-Keap1 (mouse) was a gift from Michele Pagano (Addgene plasmid # 236421 ; http://n2t.net/addgene:236421 ; RRID:Addgene_236421) -
For your References section:
EMSY inhibits homologous recombination repair and the interferon response, promoting lung cancer immune evasion. Marzio A, Kurz E, Sahni JM, Di Feo G, Puccini J, Jiang S, Hirsch CA, Arbini AA, Wu WL, Pass HI, Bar-Sagi D, Papagiannakopoulos T, Pagano M. Cell. 2022 Jan 6;185(1):169-183.e19. doi: 10.1016/j.cell.2021.12.005. Epub 2021 Dec 27. 10.1016/j.cell.2021.12.005 PubMed 34963055