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pET-30a-NSP14-SARS-CoV-2
(Plasmid #236423)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236423 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-30a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NSP14
  • Species
    SARS-CoV-2
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-30a-NSP14-SARS-CoV-2 was a gift from Michele Pagano (Addgene plasmid # 236423 ; http://n2t.net/addgene:236423 ; RRID:Addgene_236423)

  • For your References section:

    The NSP14/NSP10 RNA repair complex as a Pan-coronavirus therapeutic target. Rona G, Zeke A, Miwatani-Minter B, de Vries M, Kaur R, Schinlever A, Garcia SF, Goldberg HV, Wang H, Hinds TR, Bailly F, Zheng N, Cotelle P, Desmaele D, Landau NR, Dittmann M, Pagano M. Cell Death Differ. 2022 Feb;29(2):285-292. doi: 10.1038/s41418-021-00900-1. Epub 2021 Dec 3. 10.1038/s41418-021-00900-1 PubMed 34862481
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