pET-30a-NSP10-SARS-CoV-2
(Plasmid
#236426)
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Purposebacterial protein expression of NSP10
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236426 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-30a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNSP10
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SpeciesSARS-CoV-2
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Entrez GeneORF1ab (a.k.a. GU280_gp01)
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-30a-NSP10-SARS-CoV-2 was a gift from Michele Pagano (Addgene plasmid # 236426 ; http://n2t.net/addgene:236426 ; RRID:Addgene_236426) -
For your References section:
The NSP14/NSP10 RNA repair complex as a Pan-coronavirus therapeutic target. Rona G, Zeke A, Miwatani-Minter B, de Vries M, Kaur R, Schinlever A, Garcia SF, Goldberg HV, Wang H, Hinds TR, Bailly F, Zheng N, Cotelle P, Desmaele D, Landau NR, Dittmann M, Pagano M. Cell Death Differ. 2022 Feb;29(2):285-292. doi: 10.1038/s41418-021-00900-1. Epub 2021 Dec 3. 10.1038/s41418-021-00900-1 PubMed 34862481