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Addgene

NLS-Cherry-PCNA-P2A-Venus-E2F2
(Plasmid #236447)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236447 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLIB
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E2F2
  • Species
    H. sapiens (human)
  • Entrez Gene
    E2F2 (a.k.a. E2F-2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NLS-Cherry-PCNA-P2A-Venus-E2F2 was a gift from Michele Pagano (Addgene plasmid # 236447 ; http://n2t.net/addgene:236447 ; RRID:Addgene_236447)
  • For your References section:

    Cyclin F Controls Cell-Cycle Transcriptional Outputs by Directing the Degradation of the Three Activator E2Fs. Clijsters L, Hoencamp C, Calis JJA, Marzio A, Handgraaf SM, Cuitino MC, Rosenberg BR, Leone G, Pagano M. Mol Cell. 2019 Jun 20;74(6):1264-1277.e7. doi: 10.1016/j.molcel.2019.04.010. Epub 2019 May 23. 10.1016/j.molcel.2019.04.010 PubMed 31130363