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Addgene

pcDNA3.1alphaFEM1BalphaFLAG
(Plasmid #236461)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236461 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FEM1B
  • Species
    H. sapiens (human)
  • Entrez Gene
    FEM1B (a.k.a. F1A-ALPHA, F1AA, FEM1-beta)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1alphaFEM1BalphaFLAG was a gift from Michele Pagano (Addgene plasmid # 236461 ; http://n2t.net/addgene:236461 ; RRID:Addgene_236461)

  • For your References section:

    FEM1 proteins are ancient regulators of SLBP degradation. Dankert JF, Pagan JK, Starostina NG, Kipreos ET, Pagano M. Cell Cycle. 2017 Mar 19;16(6):556-564. doi: 10.1080/15384101.2017.1284715. Epub 2017 Jan 24. 10.1080/15384101.2017.1284715 PubMed 28118078
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