pcDNA3-2strep-FBH1
(Plasmid
#236464)
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Purposetransient overexpression of FBH1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236464 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFBH1
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SpeciesH. sapiens (human)
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Entrez GeneFBXO18 (a.k.a. RP11-536K7.6, FBH1, Fbx18)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-2strep-FBH1 was a gift from Michele Pagano (Addgene plasmid # 236464 ; http://n2t.net/addgene:236464 ; RRID:Addgene_236464) -
For your References section:
FBH1 promotes DNA double-strand breakage and apoptosis in response to DNA replication stress. Jeong YT, Rossi M, Cermak L, Saraf A, Florens L, Washburn MP, Sung P, Schildkraut CL, Pagano M. J Cell Biol. 2013 Jan 21;200(2):141-9. doi: 10.1083/jcb.201209002. Epub 2013 Jan 14. 10.1083/jcb.201209002 PubMed 23319600