pcDNA3 FLAG RRM2
(Plasmid
#236465)
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Purposetransient overexpression of RRM2 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236465 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRRM2
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SpeciesH. sapiens (human)
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Entrez GeneRRM2 (a.k.a. C2orf48, R2, RR2, RR2M)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3 FLAG RRM2 was a gift from Michele Pagano (Addgene plasmid # 236465 ; http://n2t.net/addgene:236465 ; RRID:Addgene_236465) -
For your References section:
Cyclin F-mediated degradation of ribonucleotide reductase M2 controls genome integrity and DNA repair. D'Angiolella V, Donato V, Forrester FM, Jeong YT, Pellacani C, Kudo Y, Saraf A, Florens L, Washburn MP, Pagano M. Cell. 2012 May 25;149(5):1023-34. doi: 10.1016/j.cell.2012.03.043. 10.1016/j.cell.2012.03.043 PubMed 22632967