pET32a-Nb11-P2
(Plasmid
#236499)
-
PurposeExpresses anti-Apolipoprotein A1 nanobody Nb11 as a fusion protein with coiled coil P2 in bacteria.
-
Depositing Lab
-
Publication
-
Sequence Information
-
Sequences (1) — Accept Affinity Reagent Sequence Policy
-
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 236499 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepET32a(+)
-
Backbone manufacturerNovagen
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNb11-P2
-
Insert Size (bp)543
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.03.01.640988 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pET32a-Nb11-P2 was a gift from Alexander Green (Addgene plasmid # 236499 ; http://n2t.net/addgene:236499 ; RRID:Addgene_236499) -
For your References section:
A Rapid and Modular Nanobody Assay for Plug-and-Play Antigen Detection. Kang NR, Biondo JR, Sharpes CE, Rhea KA, Garden PM, Montezco JJJ, Ringaci A, Grinstaff MW, Phillips DA, Miklos AE, Green AA. bioRxiv 2025.03.01.640988 10.1101/2025.03.01.640988