pET32a-Nb19-P4S
(Plasmid
#236500)
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PurposeExpresses anti-Apolipoprotein A1 nanobody Nb19 as a fusion protein with coiled coil P4S in bacteria.
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Depositing Lab
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Publication
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236500 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepET32a(+)
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNb19-P4S
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Insert Size (bp)549
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.03.01.640988 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET32a-Nb19-P4S was a gift from Alexander Green (Addgene plasmid # 236500 ; http://n2t.net/addgene:236500 ; RRID:Addgene_236500) -
For your References section:
A Rapid and Modular Nanobody Assay for Plug-and-Play Antigen Detection. Kang NR, Biondo JR, Sharpes CE, Rhea KA, Garden PM, Montezco JJJ, Ringaci A, Grinstaff MW, Phillips DA, Miklos AE, Green AA. bioRxiv 2025.03.01.640988 10.1101/2025.03.01.640988