Strep-His-Tev-hCGN
(Plasmid
#236512)
-
PurposeFor expression of Cingulin (human) in insect cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 236512 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonePACEBAC1
-
Vector typeInsect Expression
Growth in Bacteria
-
Bacterial Resistance(s)Gentamicin, 10 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCGN
-
SpeciesH. sapiens (human)
-
Insert Size (bp)3609
-
MutationWT
-
Entrez GeneCGN
-
Tag
/ Fusion Protein
- 2xStrep-10xHis-Tev (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn1 (unknown if destroyed)
- 3′ cloning site Xba1 (unknown if destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
S2517 (Plasmid collection number)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Strep-His-Tev-hCGN was a gift from Sandra Citi (Addgene plasmid # 236512 ; http://n2t.net/addgene:236512 ; RRID:Addgene_236512) -
For your References section:
Cingulin and paracingulin tether myosins-2 to junctions to mechanoregulate the plasma membrane. Rouaud F, Huang W, Flinois A, Jain K, Vasileva E, Di Mattia T, Mauperin M, Parry DAD, Dugina V, Chaponnier C, Mean I, Montessuit S, Mutero-Maeda A, Yan J, Citi S. J Cell Biol. 2023 Jul 3;222(7):e202208065. doi: 10.1083/jcb.202208065. Epub 2023 May 19. 10.1083/jcb.202208065 PubMed 37204781