Strep-His-Tev-GFP-hCGNL1
(Plasmid
#236521)
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PurposeFor expression of Paracingulin (human) in insect cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236521 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACEBAC1
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCGNL1
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SpeciesH. sapiens (human)
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Insert Size (bp)3906
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MutationWT
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Entrez GeneCGNL1 (a.k.a. JACOP, FLJ14957, KIAA1749, MGC138254)
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Tag
/ Fusion Protein
- 2xStrep-10xHis-Tev-EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn1 (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
S2962 (Plasmid collection number)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Strep-His-Tev-GFP-hCGNL1 was a gift from Sandra Citi (Addgene plasmid # 236521 ; http://n2t.net/addgene:236521 ; RRID:Addgene_236521) -
For your References section:
Evidence for an interaction of paracingulin with microtubules. Flinois A, Mutero-Maeda A, Montessuit S, Citi S. MicroPubl Biol. 2024 Oct 14;2024:10.17912/micropub.biology.001341. doi: 10.17912/micropub.biology.001341. eCollection 2024. 10.17912/micropub.biology.001341 PubMed 39469042