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Strep-Tev-Avi-hZO-1
(Plasmid #236527)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236527 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACEBAC1
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TJP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6000
  • Mutation
    WT
  • Entrez Gene
    TJP1 (a.k.a. ZO-1)
  • Tags / Fusion Proteins
    • StrepTag-Tev-AviTag (N terminal on insert)
    • SPY Tag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

S1928 (Plasmid collection number)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Strep-Tev-Avi-hZO-1 was a gift from Sandra Citi (Addgene plasmid # 236527 ; http://n2t.net/addgene:236527 ; RRID:Addgene_236527)

  • For your References section:

    Tension-Dependent Stretching Activates ZO-1 to Control the Junctional Localization of Its Interactors. Spadaro D, Le S, Laroche T, Mean I, Jond L, Yan J, Citi S. Curr Biol. 2017 Dec 18;27(24):3783-3795.e8. doi: 10.1016/j.cub.2017.11.014. Epub 2017 Dec 5. 10.1016/j.cub.2017.11.014 PubMed 29199076
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