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Addgene

Myc-hZO-1-HA
(Plasmid #236528)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236528 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TJP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5200
  • Mutation
    WT
  • Entrez Gene
    TJP1 (a.k.a. ZO-1)
  • Tags / Fusion Proteins
    • Myc (N terminal on insert)
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

S1947 (Plasmid collection number)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Myc-hZO-1-HA was a gift from Sandra Citi (Addgene plasmid # 236528 ; http://n2t.net/addgene:236528 ; RRID:Addgene_236528)

  • For your References section:

    Tension-Dependent Stretching Activates ZO-1 to Control the Junctional Localization of Its Interactors. Spadaro D, Le S, Laroche T, Mean I, Jond L, Yan J, Citi S. Curr Biol. 2017 Dec 18;27(24):3783-3795.e8. doi: 10.1016/j.cub.2017.11.014. Epub 2017 Dec 5. 10.1016/j.cub.2017.11.014 PubMed 29199076
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