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Addgene

pcDNA3.1(+)-FLAG-RGS14
(Plasmid #236567)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236567 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1+
  • Backbone manufacturer
    Thermo Fisher (Invitrogen)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Homo sapiens regulator of G-protein signaling 14
  • Alt name
    RGS14
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_006480.5
  • Entrez Gene
    RGS14
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cDNA Resource Center (https://www.cdna.org/home.php?cat=0)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)-FLAG-RGS14 was a gift from John Hepler (Addgene plasmid # 236567 ; http://n2t.net/addgene:236567 ; RRID:Addgene_236567)

  • For your References section:

    RGS14 regulates PTH- and FGF23-sensitive NPT2A-mediated renal phosphate uptake via binding to the NHERF1 scaffolding protein. Friedman PA, Sneddon WB, Mamonova T, Montanez-Miranda C, Ramineni S, Harbin NH, Squires KE, Gefter JV, Magyar CE, Emlet DR, Hepler JR. J Biol Chem. 2022 May;298(5):101836. doi: 10.1016/j.jbc.2022.101836. Epub 2022 Mar 17. 10.1016/j.jbc.2022.101836 PubMed 35307350
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