pMEDS
(Plasmid #236568)

• Purpose: Introduces a multiple expression and dual selection (MEDS) system to use alongside CRISPR for applications such as gene editing.
• Depositing Lab: Shannon Moonah
• Depositing Organization: University of Florida
  Located in the United States of America
• Publication: Sharma et al PLoS One. 2025 May 6;20(5):e0321881. doi: 10.1371/journal.pone.0321881. eCollection 2025.

Backbone

• Vector backbone: pMA-RQ
• Backbone size w/o insert (bp): 1357
• Total vector size (bp): 5683
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418) ; cytosine deaminase/uracil phosphoribosyltransferase (select with 5-FC)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: CD/UPRT (negative selection)
• Alt name: cytosine deaminase/uracil phosphoribosyltransferase (CD/UPRT)
• Insert Size (bp): 1125
• Tag / Fusion Protein:
  • 3x Myc

Gene/Insert 2

• Gene/Insert name: NPT II (positive selection)
• Alt name: Neomycin Phosphotransferase II
• Insert Size (bp): 792
• Tag / Fusion Protein:
  • FLAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 236568-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 236568-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.