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Addgene

pMK435
(Plasmid #236573)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236573 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    T-DNA
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.07.17.603964 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMK435 was a gift from Steven Jacobsen (Addgene plasmid # 236573 ; http://n2t.net/addgene:236573 ; RRID:Addgene_236573)
  • For your References section:

    Viral delivery of an RNA-guided genome editor for transgene-free germline editing in Arabidopsis. Weiss T, Kamalu M, Shi H, Li Z, Amerasekera J, Zhong Z, Adler BA, Song MM, Vohra K, Wirnowski G, Chitkara S, Ambrose C, Steinmetz N, Sridharan A, Sahagun D, Banfield JF, Doudna JA, Jacobsen SE. Nat Plants. 2025 Apr 22. doi: 10.1038/s41477-025-01989-9. 10.1038/s41477-025-01989-9 PubMed 40263581