pMK435
(Plasmid
#236573)
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Purpose(Empty Backbone) Cloning vector for PaqCI Golden Gate assembly of ISYmu1 gRNAs into TRV2 Architecture_B
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 236573 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneT-DNA
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.07.17.603964 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMK435 was a gift from Steven Jacobsen (Addgene plasmid # 236573 ; http://n2t.net/addgene:236573 ; RRID:Addgene_236573) -
For your References section:
Viral delivery of an RNA-guided genome editor for transgene-free germline editing in Arabidopsis. Weiss T, Kamalu M, Shi H, Li Z, Amerasekera J, Zhong Z, Adler BA, Song MM, Vohra K, Wirnowski G, Chitkara S, Ambrose C, Steinmetz N, Sridharan A, Sahagun D, Banfield JF, Doudna JA, Jacobsen SE. Nat Plants. 2025 Apr 22. doi: 10.1038/s41477-025-01989-9. 10.1038/s41477-025-01989-9 PubMed 40263581