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pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 1
(Plasmid #236618)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236618 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCAG-puro-3xFLAG (DEST)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ANKRD11
  • Species
    H. sapiens (human)
  • Mutation
    aa 1-658
  • Entrez Gene
    ANKRD11 (a.k.a. ANCO-1, ANCO1, LZ16, T13)
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on backbone)
    • 3xHA (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 1 was a gift from Tadashi Nakagawa (Addgene plasmid # 236618 ; http://n2t.net/addgene:236618 ; RRID:Addgene_236618)

  • For your References section:

    KBG syndrome-associated protein ANKRD11 regulates SETD5 expression to modulate rRNA levels and translation. Sashiyama S, Nakagawa T, Nakagawa M, Hosogane M, Watanabe Y, Ashitomi H, Yamane K, Shibuya N, Moroishi T, Nakayama K, Hosoi T. iScience. 2025 May 19;28(6):112699. doi: 10.1016/j.isci.2025.112699. eCollection 2025 Jun 20. 10.1016/j.isci.2025.112699 PubMed 40520101
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